Features
- 3M Attest Rapid Readout Biological Indicator For Steam detects the presence of Geobacillus stearothermophilus by detecting the activity of alpha‑glucosidase, an enzyme present within the organism
- Presence of the enzyme is detected by reading fluorescence produced by the enzymatic breakdown of a non‑fluorescent substrate, this creates a fluorescence change, which is detected by the auto‑reader
- Fluorescence change indicates a steam sterilization process failure
- Presence of G. stearothermophilus organisms by a visual color change reaction
- Biochemical activity of the G. stearothermophilus organism produces acid by‑products that cause the media to change color from purple to yellow
- Visual pH color change also indicates a steam sterilization process failure
- Due to the high sensitivity of the 3‑hour fluorescent results, however, there is no advantage to incubating the Attest 1292 Rapid Readout Biological Indicator beyond 3 hours
- Provides results in three hours
- Compliant with ANSI/AAMI/ISO 11138:2006 Parts 1 and 3
- Self-contained, reducing the risk of false positives due to contamination
- Automatic readout means unambiguous YES/NO, PASS/FAIL result
- Indications: Use the Attest™ 1292 Rapid Readout Biological Indicator to monitor:
– 250°F (121°C) gravity steam sterilization cycles
– 270°F (132°C) vacuum assisted steam sterilization cycles - Click Here for User Manual
More Information
- Storage:
– Best stored in the original box under normal room conditions: 15-30ºC (59-86ºF) and 35-60% relative humidity.
– Do not store these biological indicators near sterilants or other chemicals. - Precautions:
Do not use the Attest™ 1292 Rapid Readout Biological Indicator to monitor:
– 270°F (132°C) gravity steam sterilization cycles.
– Dry heat, chemical vapor, ethylene oxide or other low temperature sterilization processes. - Warning:
– There is a glass ampoule inside the plastic vial of the biological indicator. To avoid the risk of serious injury from flying debris due to a ruptured biological indicator:
– Allow the biological indicator to cool for the recommended time period before crushing. Crushing or excessive handling of the biological indicator before cooling may cause the glass ampoule to burst.
– Wear safety glasses and gloves when removing the biological indicator from the sterilizer.
– Wear safety glasses when crushing the biological indicator.
– Handle the biological indicator by the cap when crushing and tapping.
– Do not use your fingers to crush the glass ampoule.
– Do not roll the biological indicator between fingers to wet the spore strip






